FRESH WATER
AQUACULTURE TEST KIT
MODEL AQ-3 CODE 3634-03
NOTE: It is important to read the instruction manual
before attempting to perform the tests with the short
form instructions provided below.
*WARNING: Reagents marked with a * are considered
hazardous substances. Material Safety Data Sheets (MSDS)
are supplied for these reagents. For your safety, read label
and accompanying MSDS before using.
To order individual reagents or test kit components, use the
specified code number.
AMMONIA NITROGEN
1. Fill test tube (0106) to 5 mL line with sample water.
2. Add 4 drops Ammonia Nitrogen Reagent #1
(4797WT). Cap and mix. Wait 1 minute.
3. Add 12 drops of *Ammonia Nitrogen Reagent #2
(4798WT). Cap and mix. Wait 5 minutes.
4. Insert test tube into Octa-Slide Viewer (1100) with
Ammonia Nitrogen Octa-Slide Bar (3438) inserted.
5. Record as ppm Ammonia Nitrogen (NH
3
–
N).
NITRITE NITROGEN
1. Fill test tube (0106) to 2.5 mL line with sample water.
2. Dilute to 5 mL line with *Mixed Acid Reagent
(V-6278).
3. Use 0.1g spoon (0699) to add 0.1g of *Color
Developing Reagent (V-6281). Cap and mix for 1
minute. Wait 5 minutes.
4. Insert test tube into Octa-Slide Viewer (1100) with
Nitrite Nitrogen Octa-Slide Bar (3437) inserted.
5. Record as ppm Nitrite Nitrogen (NO
2
–
N).
ALKALINITY
1.
Fill titration tube (0726) to 5 mL line with sample
water.
2. Add 4 drops of *BCG-MR Indicator (2311-EG). Cap
and mix. Sample will turn blue-green.
@# = 3.Fill Direct Reading Titrator (0382) with *Alkalinity
Titration Reagent B (4493DR).
4. Titrate sample until blue-green color changes to pink.
5. Record as ppm Alkalinity (CaCO
3
).
CARBON DIOXIDE
1. Fill titration tube (0726) to 20 mL line with sample
water.
2. Add 2 drops *Phenolphthalein Indicator, 1% (2246). If
sample turns red, no free carbon dioxide is present. If
colorless, proceed to Step 3.
3. Fill Direct Reading Titrator (0380) with *Carbon
Dioxide Reagent B (4253DR).
4. Titrate sample until faint pink color persists for 30
seconds.
5. Record as ppm Carbon Dioxide (CO
2
).
CHLORIDE
1. Fill titration tube (0726) to 15 mL line with sample
water.
2. Add 1 drop *Phenolphthalein Indicator, 1% (2246). If
sample is colorless, proceed to Step 3. If sample turns
pink, add *Sulfuric Acid, 0.5N (6090) one drop at a
time until pink color disappears.
3. Add 3 drops *Chloride Reagent #1 (4504). Cap and
mix. Sample will turn yellow.
4. Fill Direct Reading Titrator (0382) with *Chloride
Reagent #2 (4505DR).
5. Titrate sample until yellow color first changes to orange
or orange-red.
6. Record as ppm Chloride (Cl).
HARDNESS
1. Fill titration tube (0726) to 12.9 mL line with sample
water.
2. Add 5 drops of *Hardness Reagent #5 (4483). Cap and
mix.
3. Add 5 drops of Hardness Reagent #6 (4485). Cap and
mix. Sample will turn red.
4. Fill Direct Reading Titrator (0382) with Hardness
Reagent #7 (4487DR).
5. Titrate sample until red color changes to clear blue.
6. Record as ppm Total Hardness (CaCO
3
).
LaMOTTE COM PANY PO BOX 329 ¨ CHESTERTOWN ¨ MARY LAND ¨ 21620
8/98
(16 pages)
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