Lamotte EZ 3-0034 User Manual

ColiQuant EZCode 3-0034Quantity Contents10 bottles Coliscan Easygel® Easygel®, 20 mL10 Petri dishes, pretreated, with lids, sterile10 Collection bottl

such as dogs, cats, birds, mammals, and people. E. coli belongs to both groups ofbacteria that are reported in water quality studies; E. coli is a fec

Non-Coliforms bacteria that form colonies that are not blue/purple orpink/magenta on Coliscan Easygel medium and are not considered to be of fecal ori

Ad di tional Re sourcesProtecting Our Watershed (LaMotte product code 5-0093) Community Action Package How to Manage water pollution, teacher guide,

• Ensure a safe field-testing experience by using the following: safety glasses foreach student, clean pail or bucket for washing hands, jug of clean

bulb to draw the water into the dropper. Carefully squeeze the bulb to expelexcess water until the dropper is filled to the desired sample volume.Use

11. Colonies will begin to appear after 24 hours. The best time to count colonies isafter 30 hours of incubation. Colonies should not be counted after

Dis posalAny materials containing living or viable microbes should be disinfected or sterilizedbefore being discarded. Before disposal in normal trash

has moved around the surface. Place the lid back on the dish and incubate for 48hours. Disinfect the forceps (alcohol pad, dilute household bleach, so

Differentiating Coliforms and E. coli• Colonies that have a blue-green teal color are not exhibiting any ß-galactosidase activity (which is evidenced

In ter pre ta tion of Re sults• Non-fecal coliforms are widely distributed in nature, being found both asnaturally occurring soil organisms, and i

Water Sampling• The sample is collected while facing upstream to avoid contamination from the hand holding the bottle. Wearing protective gloves will